Rafael Nadal had multiple injections during the tournament to keep him going as he won his 14th title at Roland Garros.
In what concluded in a dominating victory for Charles Leclerc, there were significant other stories that unfurled throughout.
The injury blow will affect his build-up up to the next Grand Slam of the year - the French Open. The Roland Garros takes place from May 22nd to June 5th.
After the tournament was cancelled in 2020, Barty returned in 2021 and successfully defended her title.
The former World No. 31 had famously knocked out Swiss great Roger Federer from the Wimbledon in 2013.
The Russian, who was a finalist at the Australian Open 2022, will be officially crowned the new men's No.1 on Monday.
The finish time of 4:55 am marked the latest ever finish to an ATP match. It has beaten Lleyton Hewitt’s clash with Marcos Baghdatis in the 2008 Australian Open.
The World No.1 will feature in Duty Free Tennis Championships after being deported from Australia ahead of the first Grand Slam of the Year.
The 22-year-old, who dropped just eight points on serve in eight service games, registered a comfortable 6-4, 6-0 win over the World No. 73 Slovak in just 52 minutes.
In 2011, Popyrin won the Australian u12s Grasscourt Championships and, with Chase Ferguson, won the u12s National Claycourt Doubles.