Manchester City have been impressive in both domestic and European matches this season, making them the clear favourites on Wednesday night.
Sitting second in the Premier League table, Manchester City have 29 points from 12 games. They are two points shy of leaders Arsenal in the standings.
Viktoria Plzen will be looking for vengeance against a heartbroken Barcelona side after suffering a humiliating 5-1 defeat to Xavi's men.
Napoli are topping both Serie A and their Champions League groups. They have 32 points from 12 games in the league.
These two sides faced each other on Matchday 1 in their group and it was Bayern Munich who won that match 2-0 in Milan.
The surprise Serie A leaders have won all their matches in this group and have a chance to make it six out of six on Tuesday.
Inter Milan needed all three points against Viktoria Plzen in their last match to clinch the knockout ticket and the Italians did it with style in a 4-0 win.
The Reds are currently placed eight in the standings, with 16 points from 11 games. They have won only four of those matches.
After beating Rangers convincingly in their opening group match, Ajax lost their next three games on the bounce.
Sitting second in Group A, Jurgen Klopp’s side have nine points from four matches. After losing their opener against Napoli, they have won three on the bounce.