On the back of her early Rome exit, Serena is set to continue her clay preparation at Emilia Romagna Open in Parma, Italy.
Pliskova will play reigning Roland Garros champion Iga Swiatek for the title in Rome.
The American teenager advanced to her first career WTA 1000-level semifinal in Rome after the World No.1 retired in the second set.
Pliskova rallied from a set down to beat Gauff; No.11 seed Jennifer Brady had it easy against Venus Williams.
Coco Gauff has made rapid strides in Tennis already at the age of 17 and the future looks bright for this American teenager.