Van de Zandschulp led 4-3 in the first set, and Rune was serving at 40/15 when he quit due to a sore chest.
The Norwegian's defeat came at the hands of Dutch player and eighth seed Botic van de Zandschulp.
Home favourite and top seed Alexander Zverev would definitely want to forget about Wednesday as he fell to a shocking defeat against Norway's Holger Rune.
Daniil Medvedev beat Botic van de Zandschulp 6-4, 6-4, 6-2 to make it into the fourth round of the Australian Open 2022.