The last meeting between these two teams in a major competition was at the 2010 World Cup, in which Germany defeated England 4-1.
Iceland pulled off the greatest result in their history, coming from behind to stun England and reach the Euro 2016 quarter-finals.
Southgate chose players in the group stage who were happy to receive the ball at their feet but were hesitant to stretch the opposition's defence with their movements.
Arsenal winger Bukayo Saka played a starring role as England edged past Czech Republic 1-0 to reach the knockout stages as group leaders.
Raheem Sterling's early header helps the Three Lions register a narrow victory over the Czech Republic to secure the top spot in Group D.
England beat Czech Republic 1-0 at Wembley on Tuesday to go through to the last 16 of Euro 2020 as Group D winners courtesy of Raheem Sterling's 12th minute goal.
Tottenham Hotspur forward Kane has been substituted in all two of England's Euro 2020 group stage matches due to disappointing performances.
England manager Gareth Southgate made it clear that Harry Kane will start the Euro 2020 game against Czech Republic.
Raheem Sterling's goal ensured England won their opening game in an edition of the Euros for the first time in their history.
Croatia's defeat was their first in their opening group match at a UEFA European Championship.