Tuchel confirmed the trio he was not able to call upon at late notice were Cesar Azpilicueta, Ruben Loftus-Cheek and Andreas Christensen.
The Danish international has become an important player under Tuchel, as Chelsea continue to build on their positive start to the season.
Chelsea were at their ruthless best on Saturday, scoring seven times without a reply against Norwich City in the Premier League.
Chelsea trounced Malmo 4-0 in Champions League, but the victory was soured a bit as Lukaku and Werner were both forced off with injuries before half-time.
The Chelsea Academy graduate has often played higher up the pitch, but Thomas Tuchel has deployed him in a deeper role this season.
Goalkeeper Mendy and left-back Chilwell played starring roles as Chelsea edged past Brentford in their Premier League clash.
Chelsea beat Brentford 1-0 on Saturday at Brentford Community stadium courtesy of Ben Chilwell’s first half strike and goalkeeper Edouard Mendy’s outstanding performance.
The game will come too early for the return of Christian Pulisic, while Antonio Rudiger picked up an injury on international duty.
The 22-year-old has been nominated for the award for the first time in his career, after playing an influential role in Chelsea winning the Champions League last season.
Tammy Abraham has been in fine form for Roma, making his presence felt with fine performances.