England will be aiming to reach their second consecutive last-4 in a major tournament whereas Ukraine will be hoping to make their first ever.
Kane ended his barren run at Euro 2020, netting the second goal against Germany in the last-16, which was assisted by Grealish.
The Manchester City winger has been enjoying a dream run at Euro 2020, emerging as England’s top goal scorer with three strikes.
Two late goals from Raheem Sterling and Harry Kane helped England beat Germany in the round-of-16 of Euro 2020.
With the likes of France and Portugal already out of the competition, Southgate will be looking to write his name in the history books.
Portugal allowed German wing-backs complete freedom in their 2-4 defeat, which England must avoid in their Euro 2020 last-16 clash.
The last meeting between these two teams in a major competition was at the 2010 World Cup, in which Germany defeated England 4-1.
England manager Gareth Southgate believes their Euro 2020 match against Germany is a good chance for the team to 'make some history'.
Southgate chose players in the group stage who were happy to receive the ball at their feet but were hesitant to stretch the opposition's defence with their movements.
Gareth Southgate is rated so highly that the English Football Association already want to extend his managerial contract beyond 2022.